rabbit anti-mouse il-1β Search Results


rabbit anti arpc5  (Novus Biologicals)
92
Novus Biologicals rabbit anti arpc5
Rabbit Anti Arpc5, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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hcn4  (Alomone Labs)
96
Alomone Labs hcn4
Hcn4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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anti il 1β 3a6 mouse mab  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc anti il 1β 3a6 mouse mab
Fig. 4. Salidroside (SAL) alleviates UVB induced inflammasome activation and reduces the release of inflammatory factors. (A) SAL inhibited the expressions of NLRP3, Caspase1, C-Caspase1 and GSDMD-N. (B) Concentration <t>of</t> <t>IL-1β</t> in supernatants of the culture media. (C) Concentration of IL-18 in supernatants of the culture media. All data were displayed as mean ± SEM, n = 5. *P < 0.05, **P < 0.01 versus group of UVB.
Anti Il 1β 3a6 Mouse Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti il 1β 3a6 mouse mab/product/Cell Signaling Technology Inc
Average 98 stars, based on 1 article reviews
anti il 1β 3a6 mouse mab - by Bioz Stars, 2026-03
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mouse monoclonal anti il 1β  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology mouse monoclonal anti il 1β
Fig. 4. Salidroside (SAL) alleviates UVB induced inflammasome activation and reduces the release of inflammatory factors. (A) SAL inhibited the expressions of NLRP3, Caspase1, C-Caspase1 and GSDMD-N. (B) Concentration <t>of</t> <t>IL-1β</t> in supernatants of the culture media. (C) Concentration of IL-18 in supernatants of the culture media. All data were displayed as mean ± SEM, n = 5. *P < 0.05, **P < 0.01 versus group of UVB.
Mouse Monoclonal Anti Il 1β, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti il 1β/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
mouse monoclonal anti il 1β - by Bioz Stars, 2026-03
96/100 stars
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mouse anti plzf mab  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology mouse anti plzf mab
Fig. 3 G410 and G467 of <t>PLZF</t> are critical for its binding to CRBN in the presence of pomalidomide. a Schematic representation of the ZF motifs of PLZF. b Multiple sequence alignment showing PLZF ZF motifs along with validated ZF degrons from Ikaros, ZFP91, <t>and</t> <t>SALL4.</t> Cys and His residues (blue and green, respectively) comprising ZF motifs and critical Gly residues (red) are highlighted. c FLAG-HA (FH)-CRBN and Myc-FLAG (MF)-PLZF carrying point mutations were coexpressed in 293T cells and immunoprecipitated with anti-HA antibody in the presence or absence of pomalidomide. d Schematic representation of EGFP-fused PLZF ZF proteins. e FH-CRBN and EGFP-fused PLZF ZF proteins were coexpressed in 293T cells and immunoprecipitated with anti-Flag antibody in the presence or absence of pomalidomide. WCL whole-cell lysate. All experiments were conducted more than twice with similar results.
Mouse Anti Plzf Mab, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
mouse anti plzf mab - by Bioz Stars, 2026-03
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rabbit anti 14 3 3 pan antibody  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rabbit anti 14 3 3 pan antibody
Fig. 3 G410 and G467 of <t>PLZF</t> are critical for its binding to CRBN in the presence of pomalidomide. a Schematic representation of the ZF motifs of PLZF. b Multiple sequence alignment showing PLZF ZF motifs along with validated ZF degrons from Ikaros, ZFP91, <t>and</t> <t>SALL4.</t> Cys and His residues (blue and green, respectively) comprising ZF motifs and critical Gly residues (red) are highlighted. c FLAG-HA (FH)-CRBN and Myc-FLAG (MF)-PLZF carrying point mutations were coexpressed in 293T cells and immunoprecipitated with anti-HA antibody in the presence or absence of pomalidomide. d Schematic representation of EGFP-fused PLZF ZF proteins. e FH-CRBN and EGFP-fused PLZF ZF proteins were coexpressed in 293T cells and immunoprecipitated with anti-Flag antibody in the presence or absence of pomalidomide. WCL whole-cell lysate. All experiments were conducted more than twice with similar results.
Rabbit Anti 14 3 3 Pan Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
rabbit anti 14 3 3 pan antibody - by Bioz Stars, 2026-03
95/100 stars
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alexa fluor 488 affinipure goat anti mouse igg  (Jackson Immuno)
96
Jackson Immuno alexa fluor 488 affinipure goat anti mouse igg
Fig. 3 G410 and G467 of <t>PLZF</t> are critical for its binding to CRBN in the presence of pomalidomide. a Schematic representation of the ZF motifs of PLZF. b Multiple sequence alignment showing PLZF ZF motifs along with validated ZF degrons from Ikaros, ZFP91, <t>and</t> <t>SALL4.</t> Cys and His residues (blue and green, respectively) comprising ZF motifs and critical Gly residues (red) are highlighted. c FLAG-HA (FH)-CRBN and Myc-FLAG (MF)-PLZF carrying point mutations were coexpressed in 293T cells and immunoprecipitated with anti-HA antibody in the presence or absence of pomalidomide. d Schematic representation of EGFP-fused PLZF ZF proteins. e FH-CRBN and EGFP-fused PLZF ZF proteins were coexpressed in 293T cells and immunoprecipitated with anti-Flag antibody in the presence or absence of pomalidomide. WCL whole-cell lysate. All experiments were conducted more than twice with similar results.
Alexa Fluor 488 Affinipure Goat Anti Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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horseradish peroxidase conjugated donkey antimouse igg secondary antibody  (Jackson Immuno)
96
Jackson Immuno horseradish peroxidase conjugated donkey antimouse igg secondary antibody
Fig. 3 G410 and G467 of <t>PLZF</t> are critical for its binding to CRBN in the presence of pomalidomide. a Schematic representation of the ZF motifs of PLZF. b Multiple sequence alignment showing PLZF ZF motifs along with validated ZF degrons from Ikaros, ZFP91, <t>and</t> <t>SALL4.</t> Cys and His residues (blue and green, respectively) comprising ZF motifs and critical Gly residues (red) are highlighted. c FLAG-HA (FH)-CRBN and Myc-FLAG (MF)-PLZF carrying point mutations were coexpressed in 293T cells and immunoprecipitated with anti-HA antibody in the presence or absence of pomalidomide. d Schematic representation of EGFP-fused PLZF ZF proteins. e FH-CRBN and EGFP-fused PLZF ZF proteins were coexpressed in 293T cells and immunoprecipitated with anti-Flag antibody in the presence or absence of pomalidomide. WCL whole-cell lysate. All experiments were conducted more than twice with similar results.
Horseradish Peroxidase Conjugated Donkey Antimouse Igg Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/horseradish peroxidase conjugated donkey antimouse igg secondary antibody/product/Jackson Immuno
Average 96 stars, based on 1 article reviews
horseradish peroxidase conjugated donkey antimouse igg secondary antibody - by Bioz Stars, 2026-03
96/100 stars
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anti mouse igg  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti mouse igg
Fig. 3 G410 and G467 of <t>PLZF</t> are critical for its binding to CRBN in the presence of pomalidomide. a Schematic representation of the ZF motifs of PLZF. b Multiple sequence alignment showing PLZF ZF motifs along with validated ZF degrons from Ikaros, ZFP91, <t>and</t> <t>SALL4.</t> Cys and His residues (blue and green, respectively) comprising ZF motifs and critical Gly residues (red) are highlighted. c FLAG-HA (FH)-CRBN and Myc-FLAG (MF)-PLZF carrying point mutations were coexpressed in 293T cells and immunoprecipitated with anti-HA antibody in the presence or absence of pomalidomide. d Schematic representation of EGFP-fused PLZF ZF proteins. e FH-CRBN and EGFP-fused PLZF ZF proteins were coexpressed in 293T cells and immunoprecipitated with anti-Flag antibody in the presence or absence of pomalidomide. WCL whole-cell lysate. All experiments were conducted more than twice with similar results.
Anti Mouse Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti mouse igg/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
anti mouse igg - by Bioz Stars, 2026-03
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anti il1β  (Proteintech)
96
Proteintech anti il1β
Fig. 3 G410 and G467 of <t>PLZF</t> are critical for its binding to CRBN in the presence of pomalidomide. a Schematic representation of the ZF motifs of PLZF. b Multiple sequence alignment showing PLZF ZF motifs along with validated ZF degrons from Ikaros, ZFP91, <t>and</t> <t>SALL4.</t> Cys and His residues (blue and green, respectively) comprising ZF motifs and critical Gly residues (red) are highlighted. c FLAG-HA (FH)-CRBN and Myc-FLAG (MF)-PLZF carrying point mutations were coexpressed in 293T cells and immunoprecipitated with anti-HA antibody in the presence or absence of pomalidomide. d Schematic representation of EGFP-fused PLZF ZF proteins. e FH-CRBN and EGFP-fused PLZF ZF proteins were coexpressed in 293T cells and immunoprecipitated with anti-Flag antibody in the presence or absence of pomalidomide. WCL whole-cell lysate. All experiments were conducted more than twice with similar results.
Anti Il1β, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti il1β/product/Proteintech
Average 96 stars, based on 1 article reviews
anti il1β - by Bioz Stars, 2026-03
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anti mth1  (Novus Biologicals)
93
Novus Biologicals anti mth1
Fig. 3 G410 and G467 of <t>PLZF</t> are critical for its binding to CRBN in the presence of pomalidomide. a Schematic representation of the ZF motifs of PLZF. b Multiple sequence alignment showing PLZF ZF motifs along with validated ZF degrons from Ikaros, ZFP91, <t>and</t> <t>SALL4.</t> Cys and His residues (blue and green, respectively) comprising ZF motifs and critical Gly residues (red) are highlighted. c FLAG-HA (FH)-CRBN and Myc-FLAG (MF)-PLZF carrying point mutations were coexpressed in 293T cells and immunoprecipitated with anti-HA antibody in the presence or absence of pomalidomide. d Schematic representation of EGFP-fused PLZF ZF proteins. e FH-CRBN and EGFP-fused PLZF ZF proteins were coexpressed in 293T cells and immunoprecipitated with anti-Flag antibody in the presence or absence of pomalidomide. WCL whole-cell lysate. All experiments were conducted more than twice with similar results.
Anti Mth1, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti mth1/product/Novus Biologicals
Average 93 stars, based on 1 article reviews
anti mth1 - by Bioz Stars, 2026-03
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anti parp2  (Proteintech)
93
Proteintech anti parp2
Fig. 3 G410 and G467 of <t>PLZF</t> are critical for its binding to CRBN in the presence of pomalidomide. a Schematic representation of the ZF motifs of PLZF. b Multiple sequence alignment showing PLZF ZF motifs along with validated ZF degrons from Ikaros, ZFP91, <t>and</t> <t>SALL4.</t> Cys and His residues (blue and green, respectively) comprising ZF motifs and critical Gly residues (red) are highlighted. c FLAG-HA (FH)-CRBN and Myc-FLAG (MF)-PLZF carrying point mutations were coexpressed in 293T cells and immunoprecipitated with anti-HA antibody in the presence or absence of pomalidomide. d Schematic representation of EGFP-fused PLZF ZF proteins. e FH-CRBN and EGFP-fused PLZF ZF proteins were coexpressed in 293T cells and immunoprecipitated with anti-Flag antibody in the presence or absence of pomalidomide. WCL whole-cell lysate. All experiments were conducted more than twice with similar results.
Anti Parp2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti parp2/product/Proteintech
Average 93 stars, based on 1 article reviews
anti parp2 - by Bioz Stars, 2026-03
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Image Search Results


Fig. 4. Salidroside (SAL) alleviates UVB induced inflammasome activation and reduces the release of inflammatory factors. (A) SAL inhibited the expressions of NLRP3, Caspase1, C-Caspase1 and GSDMD-N. (B) Concentration of IL-1β in supernatants of the culture media. (C) Concentration of IL-18 in supernatants of the culture media. All data were displayed as mean ± SEM, n = 5. *P < 0.05, **P < 0.01 versus group of UVB.

Journal: Journal of Functional Foods

Article Title: Salidroside alleviates UVB-induced skin damage by inhibiting keratinocytes pyroptosis via the AQP3/ROS/GSDMD-N signaling pathway

doi: 10.1016/j.jff.2023.105647

Figure Lengend Snippet: Fig. 4. Salidroside (SAL) alleviates UVB induced inflammasome activation and reduces the release of inflammatory factors. (A) SAL inhibited the expressions of NLRP3, Caspase1, C-Caspase1 and GSDMD-N. (B) Concentration of IL-1β in supernatants of the culture media. (C) Concentration of IL-18 in supernatants of the culture media. All data were displayed as mean ± SEM, n = 5. *P < 0.05, **P < 0.01 versus group of UVB.

Article Snippet: The followings are the information of primary antibodies: rabbit anti-NLRP3 (Q96P20) mAb T55651 (1:1000, Abmart), rabbit anti-Caspase-1 (E9R2D) mAb #83383S (1:1000, CST), rabbit anti- C-Caspase-1 (Asp296) (E2G2I) Rabbit mAb #89332 (reactivity: mouse) (1:1000, CST), anti-C-Caspase-1 (Asp297) (D57A2) Rabbit mAb #4199(reactivity: human) (1:1000,CST), anti- Gasdermin D N terminal (P57764) (Q9D8T2) Rabbit mAb, PU224937(1:1000, abmart), anti-IL-1β (3A6) Mouse mAb #12242 (1:1000, CST), anti-IL-18 (E3G8R) Rabbit mAb #67775 (reactivity: human) (1:1000,CST), IL-18 (E8P5O) Rabbit mAb #57058 (reactivity: mouse) (1:1000,CST), anti-rabbit IgG (H + L) Biotinylated Antibody #14708 (1:3000,CST), anti-mouse IgG (H + L), Biotinylated Antibody #14709 (1:3000,CST).

Techniques: Activation Assay, Concentration Assay

Fig. 5. Salidroside (SAL) suppresses pyroptosis via down-regulatin AQP3/ROS/GSDMD-N signaling pathway in HaCaT cell line. (A) The bands and analysis results of AQP3 expression in HaCaT cell line after the treatment of SAL. (B) The fluorescence results of the HaCaT cells with overexpression of AQP3. (C) The bands and analysis of AQP3 expression in HaCaT cell line after overexpression. (D) qPCR results of HaCaT with AQP3 overexpression and NC. (E) The concentration of H2O2 in cells. (F) The bands and analysis of Caspase1, C-Caspase1, NLRP3 and GSDMD-N after the overexpression of AQP3. (G) The concentration of IL-18 in cells after the overexpression of AQP3. (H) The concentration of IL-1β in cells after the overexpression of AQP3. (I) The level of ROS in cells after the overexpression of AQP3. (J) The inflammasomes in cells visualized by TEM. All data were displayed as mean ± SEM, n = 3. *P < 0.05, **P < 0.01 versus group of UVB; # P < 0.05, ##P < 0.01 versus group of AQP3+/+.

Journal: Journal of Functional Foods

Article Title: Salidroside alleviates UVB-induced skin damage by inhibiting keratinocytes pyroptosis via the AQP3/ROS/GSDMD-N signaling pathway

doi: 10.1016/j.jff.2023.105647

Figure Lengend Snippet: Fig. 5. Salidroside (SAL) suppresses pyroptosis via down-regulatin AQP3/ROS/GSDMD-N signaling pathway in HaCaT cell line. (A) The bands and analysis results of AQP3 expression in HaCaT cell line after the treatment of SAL. (B) The fluorescence results of the HaCaT cells with overexpression of AQP3. (C) The bands and analysis of AQP3 expression in HaCaT cell line after overexpression. (D) qPCR results of HaCaT with AQP3 overexpression and NC. (E) The concentration of H2O2 in cells. (F) The bands and analysis of Caspase1, C-Caspase1, NLRP3 and GSDMD-N after the overexpression of AQP3. (G) The concentration of IL-18 in cells after the overexpression of AQP3. (H) The concentration of IL-1β in cells after the overexpression of AQP3. (I) The level of ROS in cells after the overexpression of AQP3. (J) The inflammasomes in cells visualized by TEM. All data were displayed as mean ± SEM, n = 3. *P < 0.05, **P < 0.01 versus group of UVB; # P < 0.05, ##P < 0.01 versus group of AQP3+/+.

Article Snippet: The followings are the information of primary antibodies: rabbit anti-NLRP3 (Q96P20) mAb T55651 (1:1000, Abmart), rabbit anti-Caspase-1 (E9R2D) mAb #83383S (1:1000, CST), rabbit anti- C-Caspase-1 (Asp296) (E2G2I) Rabbit mAb #89332 (reactivity: mouse) (1:1000, CST), anti-C-Caspase-1 (Asp297) (D57A2) Rabbit mAb #4199(reactivity: human) (1:1000,CST), anti- Gasdermin D N terminal (P57764) (Q9D8T2) Rabbit mAb, PU224937(1:1000, abmart), anti-IL-1β (3A6) Mouse mAb #12242 (1:1000, CST), anti-IL-18 (E3G8R) Rabbit mAb #67775 (reactivity: human) (1:1000,CST), IL-18 (E8P5O) Rabbit mAb #57058 (reactivity: mouse) (1:1000,CST), anti-rabbit IgG (H + L) Biotinylated Antibody #14708 (1:3000,CST), anti-mouse IgG (H + L), Biotinylated Antibody #14709 (1:3000,CST).

Techniques: Expressing, Fluorescence, Over Expression, Concentration Assay

Fig. 7. Salidroside (SAL) relieves UVB-induced oxidative damage and pyroptosis in mice (A) Immunohistochemical staining and analysis for AQP3. (B) The expression of AQP3 in the skin leision was dettected by Western blotting. (C) The concentrations of H2O2. (D) MDA, (E F) SOD, and GSH in skin lesions. (G) Immunohistochemistry staining and analysis for NLRP3. (H) The expressions of NLRP3, Caspase-1, C-Caspase-1, GSDMD-N, IL-1β and IL-18 were detected by Western blotting. All data were displayed as Mean ± SD, n = 3. *P < 0.05, **P < 0.01 versus group of UVB.

Journal: Journal of Functional Foods

Article Title: Salidroside alleviates UVB-induced skin damage by inhibiting keratinocytes pyroptosis via the AQP3/ROS/GSDMD-N signaling pathway

doi: 10.1016/j.jff.2023.105647

Figure Lengend Snippet: Fig. 7. Salidroside (SAL) relieves UVB-induced oxidative damage and pyroptosis in mice (A) Immunohistochemical staining and analysis for AQP3. (B) The expression of AQP3 in the skin leision was dettected by Western blotting. (C) The concentrations of H2O2. (D) MDA, (E F) SOD, and GSH in skin lesions. (G) Immunohistochemistry staining and analysis for NLRP3. (H) The expressions of NLRP3, Caspase-1, C-Caspase-1, GSDMD-N, IL-1β and IL-18 were detected by Western blotting. All data were displayed as Mean ± SD, n = 3. *P < 0.05, **P < 0.01 versus group of UVB.

Article Snippet: The followings are the information of primary antibodies: rabbit anti-NLRP3 (Q96P20) mAb T55651 (1:1000, Abmart), rabbit anti-Caspase-1 (E9R2D) mAb #83383S (1:1000, CST), rabbit anti- C-Caspase-1 (Asp296) (E2G2I) Rabbit mAb #89332 (reactivity: mouse) (1:1000, CST), anti-C-Caspase-1 (Asp297) (D57A2) Rabbit mAb #4199(reactivity: human) (1:1000,CST), anti- Gasdermin D N terminal (P57764) (Q9D8T2) Rabbit mAb, PU224937(1:1000, abmart), anti-IL-1β (3A6) Mouse mAb #12242 (1:1000, CST), anti-IL-18 (E3G8R) Rabbit mAb #67775 (reactivity: human) (1:1000,CST), IL-18 (E8P5O) Rabbit mAb #57058 (reactivity: mouse) (1:1000,CST), anti-rabbit IgG (H + L) Biotinylated Antibody #14708 (1:3000,CST), anti-mouse IgG (H + L), Biotinylated Antibody #14709 (1:3000,CST).

Techniques: Immunohistochemical staining, Staining, Expressing, Western Blot, Immunohistochemistry

Fig. 3 G410 and G467 of PLZF are critical for its binding to CRBN in the presence of pomalidomide. a Schematic representation of the ZF motifs of PLZF. b Multiple sequence alignment showing PLZF ZF motifs along with validated ZF degrons from Ikaros, ZFP91, and SALL4. Cys and His residues (blue and green, respectively) comprising ZF motifs and critical Gly residues (red) are highlighted. c FLAG-HA (FH)-CRBN and Myc-FLAG (MF)-PLZF carrying point mutations were coexpressed in 293T cells and immunoprecipitated with anti-HA antibody in the presence or absence of pomalidomide. d Schematic representation of EGFP-fused PLZF ZF proteins. e FH-CRBN and EGFP-fused PLZF ZF proteins were coexpressed in 293T cells and immunoprecipitated with anti-Flag antibody in the presence or absence of pomalidomide. WCL whole-cell lysate. All experiments were conducted more than twice with similar results.

Journal: Communications biology

Article Title: PLZF and its fusion proteins are pomalidomide-dependent CRBN neosubstrates.

doi: 10.1038/s42003-021-02801-y

Figure Lengend Snippet: Fig. 3 G410 and G467 of PLZF are critical for its binding to CRBN in the presence of pomalidomide. a Schematic representation of the ZF motifs of PLZF. b Multiple sequence alignment showing PLZF ZF motifs along with validated ZF degrons from Ikaros, ZFP91, and SALL4. Cys and His residues (blue and green, respectively) comprising ZF motifs and critical Gly residues (red) are highlighted. c FLAG-HA (FH)-CRBN and Myc-FLAG (MF)-PLZF carrying point mutations were coexpressed in 293T cells and immunoprecipitated with anti-HA antibody in the presence or absence of pomalidomide. d Schematic representation of EGFP-fused PLZF ZF proteins. e FH-CRBN and EGFP-fused PLZF ZF proteins were coexpressed in 293T cells and immunoprecipitated with anti-Flag antibody in the presence or absence of pomalidomide. WCL whole-cell lysate. All experiments were conducted more than twice with similar results.

Article Snippet: Rabbit anti-human CRBN65 mAb (Celgene, San Diego, CA, 1:10000 dilution, Lot# CGN-6-4-5), mouse anti-PLZF mAb (39987, Active Motif, 1:1000 dilution, Lot#05313002), mouse anti-PLZF mAb (sc-28319, Santa Cruz, 1:1000 dilution, Lot#C0618), mouse anti-SALL4 mAb (ab57577, Abcam, 1:1000 dilution, Lot#GR3198290-2), mouse anti-Vinculin mAb (ab18058, Abcam, 1:1000 dilution, Lot#GR207163-1), mouse anti-HA11 mAb (901503, BioLegend, 1:1000 dilution, Lot#b207273), mouse anti-Flag M2 mAb (F1804, SIGMA, 1:1000 dilution, Lot#SLBS3530V), rabbit anti-Myc tag (ab9106, Abcam, 1:1000 dilution), rabbit anti-GSPT1 (ab49878, Abcam, 1:1000 dilution, Lot#GR274469-4), rabbit antiCK1α mAb (ab108296, Abcam, 1:1000 dilution, Lot#GR53415-9), and rabbit antiIkaros mAb (ab26083, Abcam, 1:1000 dilution, Lot#GR250435-1), rabbit anti-GFP (ab290, Abcam, 1:1000 dilution, Lot#GR3184825-1) were used as primary antibodies.

Techniques: Binding Assay, Sequencing, Immunoprecipitation